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Nitrogen balance is a measure of nitrogen input minus nitrogen output

Nitrogen Balance = Nitrogen intake - Nitrogen loss

Nitrogen is a fundamental component of amino acids, which are the molecular building blocks of protein. Measuring nitrogen inputs and losses can be used to study protein metabolism. 

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Estimated Average Requirement EAR

Estimated Average Requirement (EAR) is the average daily nutrient intake level that is estimated to meet the requirements of half  - 50% - of the healthy individuals in a particular life stage and gender group. 

Because the needs of the other half of the population will not be met by this amount, the EAR is increased by about 20% to arrive at the RDA.

Before setting the EAR, a specific criterion of adequacy is selected, based on a careful review of the literature. When selecting the criterion, reduction of disease risk is considered along with many other health parameters. 

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